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SourceForge net perlprimer software
Perlprimer Software, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perlprimer+software/perlprimer+software/pm37778594-110-29-32
Average 90 stars, based on 1 article reviews
perlprimer software - by Bioz Stars, 2026-09
90/100 stars

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Software:

Article Title: Long-lasting impact of chitooligosaccharide application on strigolactone biosynthesis and fungal accommodation promotes arbuscular mycorrhiza in Medicago truncatula.
Article Snippet: .. Specific primers (listed in Table S3) were designed using PERLPRIMER software (http://perlprimer.sourceforge.net) on M. truncatula CDS sequences from NCBI (http://www.ncbi.nlm.nih.gov/) andM. truncatula genome database (http://www.medicagogenome. org). ..

Article Title: APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings
Article Snippet: About 0.1 μg of total RNA treated with Amplification Grade DNase I was reverse transcribed using a High Capacity cDNA Reverse Transcription kit (AB Biosystems/Thermo Fisher, Waltham, MA, USA), following the manufacturer’s protocol. .. Gene-specific primers of APY1, APY2 , and ACT2 for RT-qPCR were designed using PerlPrimer software (perlprimer.sourceforge.net). ..

Article Title: Long-lasting impact of chito-oligosaccharide application on strigolactone biosynthesis and fungal accommodation promotes arbuscular mycorrhiza in Medicago truncatula
Article Snippet: .. Specific primers (listed in Table S3) were designed using PerlPrimer software ( http://perlprimer.sourceforge.net ) on M. truncatula CDS sequences from NCBI ( http://www.ncbi.nlm.nih.gov/ ) and M. truncatula genome database ( http://www.medicagogenome.org ). .. All primers were first tested by PCR on genomic M. truncatula and F. mosseae DNA, to confirm their plant-specificity. qRT-PCR was also used to investigate the regulation of marker genes for AM fungal accommodation and early signaling, using the primers listed in Table S4. qRT-PCR analyses were done according to Rotor-Gene SYBR Green PCR Kit instructions (QIAGEN, Hilden, Germany) and were run in a final volume of 15 μl for each tube containing 7.5 μl of Rotor-Gene SYBR Green PCR Master Mix, 5.5 μl of 3 μM specific primers mix, and 10 ng of cDNA.

Article Title: Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment
Article Snippet: .. For the design of the primers target and normalizers, we used the PerlPrimer Software ( http://perlprimer.sourceforge.net ) for amplification between 80 and 150 bp. ..

Article Title: Skeletal muscle gene expression in older adults with type 2 diabetes mellitus undergoing calorie-restricted diet and recreational sports training - a randomized clinical trial.
Article Snippet: Aims: This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients.. Methods: Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m2) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D).. The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks.

Article Title: A self-gelling hydrogel based on thiolated hyaluronic acid for three-dimensional culture of ovine preantral follicles.
Article Snippet: Three-dimensional (3D) ovarian follicle culture offers a promising option for fertility preservation in patients who cannot receive ovarian tissue transplantation.. Our research evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to routinely used alginate hydrogel (ALG).. Synthesized via a carbodiimide reaction, HA-SH facilitated a self-crosslinking hydrogel through disulfide bond formation.

Article Title: Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum
Article Snippet: .. For RT-qPCR, primer sets were designed for each target gene using PerlPrimer software (perlprimer.sourceforge.net). ..

Quantitative RT-PCR:

Article Title: APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings
Article Snippet: About 0.1 μg of total RNA treated with Amplification Grade DNase I was reverse transcribed using a High Capacity cDNA Reverse Transcription kit (AB Biosystems/Thermo Fisher, Waltham, MA, USA), following the manufacturer’s protocol. .. Gene-specific primers of APY1, APY2 , and ACT2 for RT-qPCR were designed using PerlPrimer software (perlprimer.sourceforge.net). ..

Amplification:

Article Title: Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment
Article Snippet: .. For the design of the primers target and normalizers, we used the PerlPrimer Software ( http://perlprimer.sourceforge.net ) for amplification between 80 and 150 bp. ..

Real-time Polymerase Chain Reaction:

Article Title: A self-gelling hydrogel based on thiolated hyaluronic acid for three-dimensional culture of ovine preantral follicles.
Article Snippet: Three-dimensional (3D) ovarian follicle culture offers a promising option for fertility preservation in patients who cannot receive ovarian tissue transplantation.. Our research evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to routinely used alginate hydrogel (ALG).. Synthesized via a carbodiimide reaction, HA-SH facilitated a self-crosslinking hydrogel through disulfide bond formation.

Polymerase Chain Reaction:

Article Title: A self-gelling hydrogel based on thiolated hyaluronic acid for three-dimensional culture of ovine preantral follicles.
Article Snippet: Three-dimensional (3D) ovarian follicle culture offers a promising option for fertility preservation in patients who cannot receive ovarian tissue transplantation.. Our research evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to routinely used alginate hydrogel (ALG).. Synthesized via a carbodiimide reaction, HA-SH facilitated a self-crosslinking hydrogel through disulfide bond formation.



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